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substrate solution (SouthernBiotech)

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SouthernBiotech substrate solution
Substrate Solution, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pnpp+substrate/pNPP+One+Component+Microwell+Substrate/us12584920-443-7-16
Average 93 stars, based on 20 article reviews

substrate solution - by Bioz Stars, 2026-09

93/100 stars

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Article Title: Maternal immunization confers protection to the offspring against an attaching and effacing pathogen through delivery of IgG in breast milk
Article Snippet: Aliquots of the proteins were stored at −20°C until use. table ft1 table-wrap mode="anchored" t5 caption a7 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Biotin Rat anti-Mouse IgG1 BD Biosciences Cat# 553441; RRID: AB_394861 Biotin Rat anti-Mouse IgG2a BD Biosciences Cat# 553388; RRID: AB_394826 Biotin Rat anti-Mouse IgG2b BD Biosciences Cat# 553393; RRID: AB_394831 Biotin Rat anti-Mouse IgG3 BD Biosciences Cat# 553401; RRID: AB_394838 Goat anti-Mouse IgA, Human ads-AP SouthernBiotech Cat# 1040–04; RRID: AB_619826 Goat anti-Mouse IgG1, Human ads-AP SouthernBiotech Cat# 1070–04 Goat anti-Mouse IgG2a, Human ads-AP SouthernBiotech Cat# 1080–04; RRID: AB_2692322 Goat anti-Mouse IgG2b, Human ads-AP SouthernBiotech Cat# 1090–04; RRID: AB_619828 Goat anti-Mouse IgG3, Human ads-AP SouthernBiotech Cat# 1100–04 Goat anti-Mouse IgM, Human ads-AP SouthernBiotech Cat# 1020–04; RRID: AB_619829 Peroxidase AffiniPure Rabbit Anti-Mouse IgG, F(ab’) 2 fragment specific Jackson ImmunoResearch Cat# 315–035-047; RRID: AB_2340068 Bacterial and Virus Strains Citrobacter rodentium DBS120 (pCRP1::Tn5) Dr. David Schauer ( Schauer and Falkow, 1993 ) N/A Citrobacter rodentium DBS100 Δ ler Dr. José Luis Puente ( Deng et al., 2004 ) N/A Citrobacter rodentium DBS100- lux Dr. Bruce A. Vallance ( Bergstrom et al., 2010 ) N/A Citrobacter rodentium DBS100-GFP Dr. Bruce A. Vallance ( Bergstrom et al., 2010 ) N/A Escherichia coli BL21 New England Biolabs Cat# C2530H Chemicals, Peptides, and Recombinant Proteins APC-streptavidin Biolegend Cat#: 405207 Dulbecco’s Modified Eagle Medium Thermo Fisher Scientific Cat#: 11995065 EspA-6xHis-tagged This paper N/A EspB-6xHis-tagged This paper N/A Fluriso (Isofluorane) VetOne Cat#: 501017 Gentamicin Thermo Fisher Scientific Cat#: 15710064 Imject Alum Thermo Fisher Scientific Cat#: 77161 Ni-NTA Agarose QIAGEN Cat#: 30210 NP-Ovalbumin Biosearch Technologies Cat#: N-5051 Oxytocin VetOne Cat#: 501013 pNPP Substrate, Tablets SouthernBiotech Cat#: 0201–01 Thyoglicolate Broth Sigma-Aldrich Cat# 70157 6xHis-tagged-Intimin-β 385 This paper & Sinclair and O’Brien, 2004 N/A Critical Commercial Assays Quick Start Bradford Protein Assay Bio-Rad Cat#: 500–0205 Pierce ECL Western Blotting Substrate Thermo Fisher Scientific Cat#: 32106 Experimental Models: Organisms/Strains Mouse: C57BL/6J: WT The Jackson Laboratory Cat#: 000664; RRID: IMSR_JAX:000664 Mouse: FcRn-deficient ( Fcgrt −/− ): B6.129X1- Fcgrt tm1Dcr The Jackson Laboratory Cat#: 003982; RRID: IMSR_JAX:003982 Mouse: IgA-deficient ( Igha −/− ): C.129S7(B6)- Igh-2 tm1Grh Dr. Bernard Arulanandam ( Harriman et al., 1999 ) Cat#: 031020-MU; RRID: MMRRC_031020-MU Mouse: pIgR-deficient ( Pigr −/− ): B6.129P2- Pigrt m1Fejo Dr. Christiane E. Wobus ( Uren et al., 2003 ).

Incubation:

Article Title: Vaccine-induced ErbB (EGFR/HER2)-specific immunity in spontaneous canine cancer
Article Snippet: .. Alkaline phosphatase conjugated-goat anti-dog IgG antibody (Southern Biotech; Birmingham, AL) was added to wells followed by incubation with pNPP substrate. ..

Article Title: CTLA-4 antibodies and uses thereof
Article Snippet: .. Plates were washed 6 times and 50 ul/well of pNpp substrate (Southern Biotech, China) was added and incubated at room temperature, and then plates were read at 405 nm using a microplate reader (Bio-Rad). ..

Clinical Proteomics:

Article Title: Loss of TET2 increases B-1 cell number and IgM production while limiting CDR3 diversity
Article Snippet: Briefly, EIA/RIA high-binding microplates were coated with goat anti-mouse IgM, capture antibody (Southern Biotech, 1020-01). .. Mouse IgM standards (Southern Biotech, 0101-01), or plasma samples were detected with alkaline phosphatase-conjugated goat anti-mouse IgM secondary antibody (Southern Biotech, 1020-04); and pNPP substrate (Southern Biotech 0201-01). .. Absorbance measurements were analyzed with a SpectraMAX 190 microplate reader (Molecular Devices) at 405 nm.